Construct: shRNA TRCN0000360889

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001099276.2-5054s21c1
DNA Barcode:
CTTCATCATGGTGATGCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pik3c2b (240752)
Gene Description:
phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta
Transcript:
RefSeq NM_001099276.2 (CURRENT)
Match location:
Position 5054 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360889)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCATCATGGTGATGCATAT
Hairpin Sequence:
5'-CCGG-CTTCATCATGGTGATGCATAT-CTCGAG-ATATGCATCACCATGATGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCATCATGGTGATGCATATCTCGAGATATGCATCACCATGATGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCATCATGGTGATGCATATCTCGAGATATGCATCACCATGATGAAG-3'

Other clones with same target sequence:

(none)