Construct: shRNA TRCN0000360939

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207246.3-1836s21c1
DNA Barcode:
TGCGAACACTGTGCGGGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rasgrp3 (240168)
Gene Description:
RAS, guanyl releasing protein 3
Transcript:
RefSeq NM_207246.3 (NON-CURRENT)
Match location:
Position 1836 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360939)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCGAACACTGTGCGGGATTT
Hairpin Sequence:
5'-CCGG-TGCGAACACTGTGCGGGATTT-CTCGAG-AAATCCCGCACAGTGTTCGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCGAACACTGTGCGGGATTTCTCGAGAAATCCCGCACAGTGTTCGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCGAACACTGTGCGGGATTTCTCGAGAAATCCCGCACAGTGTTCGCA-3'

Other clones with same target sequence:

  1. TRCN0000422968