Construct: shRNA TRCN0000360947

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001007576.1-2423s21c1
DNA Barcode:
CATTTGCAGGAGACGTCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gucy2f (245650)
Gene Description:
guanylate cyclase 2f
Transcript:
RefSeq NM_001007576.1 (NON-CURRENT)
Match location:
Position 2423 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000360947)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTTGCAGGAGACGTCTATA
Hairpin Sequence:
5'-CCGG-CATTTGCAGGAGACGTCTATA-CTCGAG-TATAGACGTCTCCTGCAAATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTTGCAGGAGACGTCTATACTCGAGTATAGACGTCTCCTGCAAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTTGCAGGAGACGTCTATACTCGAGTATAGACGTCTCCTGCAAATG-3'

Other clones with same target sequence:

(none)