Construct: shRNA TRCN0000361004

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007439.2-4326s21c1
DNA Barcode:
ACCTAGAGGAGAATCACTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Alk (11682)
Gene Description:
anaplastic lymphoma kinase
Transcript:
RefSeq NM_007439.2 (CURRENT)
Match location:
Position 4326 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361004)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTAGAGGAGAATCACTTTA
Hairpin Sequence:
5'-CCGG-ACCTAGAGGAGAATCACTTTA-CTCGAG-TAAAGTGATTCTCCTCTAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTAGAGGAGAATCACTTTACTCGAGTAAAGTGATTCTCCTCTAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTAGAGGAGAATCACTTTACTCGAGTAAAGTGATTCTCCTCTAGGT-3'

Other clones with same target sequence:

(none)