Construct: shRNA TRCN0000361060

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007397.2-997s21c1
DNA Barcode:
ATGTCACGAGGCCTCTCATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acvr2b (11481)
Gene Description:
activin receptor IIB
Transcript:
RefSeq NM_007397.2 (NON-CURRENT)
Match location:
Position 997 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361060)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTCACGAGGCCTCTCATAC
Hairpin Sequence:
5'-CCGG-ATGTCACGAGGCCTCTCATAC-CTCGAG-GTATGAGAGGCCTCGTGACAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTCACGAGGCCTCTCATACCTCGAGGTATGAGAGGCCTCGTGACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTCACGAGGCCTCTCATACCTCGAGGTATGAGAGGCCTCGTGACAT-3'

Other clones with same target sequence:

(none)