Construct: shRNA TRCN0000361152

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008054.2-1644s21c1
DNA Barcode:
CATCGAGCGCATGAATTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fyn (14360)
Gene Description:
Fyn proto-oncogene
Transcript:
RefSeq NM_008054.2 (CURRENT)
Match location:
Position 1644 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361152)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCGAGCGCATGAATTATAT
Hairpin Sequence:
5'-CCGG-CATCGAGCGCATGAATTATAT-CTCGAG-ATATAATTCATGCGCTCGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCGAGCGCATGAATTATATCTCGAGATATAATTCATGCGCTCGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCGAGCGCATGAATTATATCTCGAGATATAATTCATGCGCTCGATG-3'

Other clones with same target sequence:

  1. TRCN0000320616