Construct: shRNA TRCN0000361187

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007937.3-3622s21c1
DNA Barcode:
GCCTTAACCTTTGAGTTTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Epha5 (13839)
Gene Description:
Eph receptor A5
Transcript:
RefSeq NM_007937.3 (CURRENT)
Match location:
Position 3622 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361187)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTAACCTTTGAGTTTATA
Hairpin Sequence:
5'-CCGG-GCCTTAACCTTTGAGTTTATA-CTCGAG-TATAAACTCAAAGGTTAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTAACCTTTGAGTTTATACTCGAGTATAAACTCAAAGGTTAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTAACCTTTGAGTTTATACTCGAGTATAAACTCAAAGGTTAAGGC-3'

Other clones with same target sequence:

(none)