Construct: shRNA TRCN0000361267

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021461.4-1008s21c1
DNA Barcode:
AGGAAGGCAAATACGAGTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mknk1 (17346)
Gene Description:
MAP kinase-interacting serine/threonine kinase 1
Transcript:
RefSeq NM_021461.4 (NON-CURRENT)
Match location:
Position 1008 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361267)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAAGGCAAATACGAGTTTC
Hairpin Sequence:
5'-CCGG-AGGAAGGCAAATACGAGTTTC-CTCGAG-GAAACTCGTATTTGCCTTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAAGGCAAATACGAGTTTCCTCGAGGAAACTCGTATTTGCCTTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAAGGCAAATACGAGTTTCCTCGAGGAAACTCGTATTTGCCTTCCT-3'

Other clones with same target sequence:

(none)