Construct: shRNA TRCN0000361283

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010407.3-756s21c1
DNA Barcode:
GACACCGTGAAGCACTATAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hck (15162)
Gene Description:
hemopoietic cell kinase
Transcript:
RefSeq NM_010407.3 (NON-CURRENT)
Match location:
Position 756 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361283)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACACCGTGAAGCACTATAAG
Hairpin Sequence:
5'-CCGG-GACACCGTGAAGCACTATAAG-CTCGAG-CTTATAGTGCTTCACGGTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACACCGTGAAGCACTATAAGCTCGAGCTTATAGTGCTTCACGGTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACACCGTGAAGCACTATAAGCTCGAGCTTATAGTGCTTCACGGTGTC-3'

Other clones with same target sequence:

(none)