Construct: shRNA TRCN0000361298

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013571.2-1245s21c1
DNA Barcode:
AGAGTCTGTCCCGTCAGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ksr1 (16706)
Gene Description:
kinase suppressor of ras 1
Transcript:
RefSeq NM_013571.2 (NON-CURRENT)
Match location:
Position 1245 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361298)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGTCTGTCCCGTCAGATAT
Hairpin Sequence:
5'-CCGG-AGAGTCTGTCCCGTCAGATAT-CTCGAG-ATATCTGACGGGACAGACTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGTCTGTCCCGTCAGATATCTCGAGATATCTGACGGGACAGACTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGTCTGTCCCGTCAGATATCTCGAGATATCTGACGGGACAGACTCT-3'

Other clones with same target sequence:

(none)