Construct: shRNA TRCN0000361324

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008746.5-2760s21c1
DNA Barcode:
GTAAGGCAGTTTGACATATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ntrk3 (18213)
Gene Description:
neurotrophic tyrosine kinase, receptor, type 3
Transcript:
RefSeq NM_008746.5 (CURRENT)
Match location:
Position 2760 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361324)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAAGGCAGTTTGACATATAT
Hairpin Sequence:
5'-CCGG-GTAAGGCAGTTTGACATATAT-CTCGAG-ATATATGTCAAACTGCCTTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAAGGCAGTTTGACATATATCTCGAGATATATGTCAAACTGCCTTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAAGGCAGTTTGACATATATCTCGAGATATATGTCAAACTGCCTTAC-3'

Other clones with same target sequence:

(none)