Construct: shRNA TRCN0000361389

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008745.2-794s21c1
DNA Barcode:
CCGGAGAACATCACGGAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ntrk2 (18212)
Gene Description:
neurotrophic tyrosine kinase, receptor, type 2
Transcript:
RefSeq NM_008745.2 (NON-CURRENT)
Match location:
Position 794 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361389)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAGAACATCACGGAAATT
Hairpin Sequence:
5'-CCGG-CCGGAGAACATCACGGAAATT-CTCGAG-AATTTCCGTGATGTTCTCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAGAACATCACGGAAATTCTCGAGAATTTCCGTGATGTTCTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAGAACATCACGGAAATTCTCGAGAATTTCCGTGATGTTCTCCGG-3'

Other clones with same target sequence:

(none)