Construct: shRNA TRCN0000361417

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008841.2-1960s21c1
DNA Barcode:
GAAATGCAGCAAGGAGTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pik3r2 (18709)
Gene Description:
phosphoinositide-3-kinase regulatory subunit 2
Transcript:
RefSeq NM_008841.2 (NON-CURRENT)
Match location:
Position 1960 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361417)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAATGCAGCAAGGAGTATTT
Hairpin Sequence:
5'-CCGG-GAAATGCAGCAAGGAGTATTT-CTCGAG-AAATACTCCTTGCTGCATTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAATGCAGCAAGGAGTATTTCTCGAGAAATACTCCTTGCTGCATTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAATGCAGCAAGGAGTATTTCTCGAGAAATACTCCTTGCTGCATTTC-3'

Other clones with same target sequence:

(none)