Construct: shRNA TRCN0000361422

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181585.5-1154s21c1
DNA Barcode:
GAGCGAATTATGATGAATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pik3r3 (18710)
Gene Description:
phosphoinositide-3-kinase regulatory subunit 3
Transcript:
RefSeq NM_181585.5 (CURRENT)
Match location:
Position 1154 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361422)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCGAATTATGATGAATTAT
Hairpin Sequence:
5'-CCGG-GAGCGAATTATGATGAATTAT-CTCGAG-ATAATTCATCATAATTCGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCGAATTATGATGAATTATCTCGAGATAATTCATCATAATTCGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCGAATTATGATGAATTATCTCGAGATAATTCATCATAATTCGCTC-3'

Other clones with same target sequence:

(none)