Construct: shRNA TRCN0000361458

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009074.2-3469s21c1
DNA Barcode:
TGTTGTCTACCACGGAGAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mst1r (19882)
Gene Description:
macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
Transcript:
RefSeq NM_009074.2 (CURRENT)
Match location:
Position 3469 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361458)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTTGTCTACCACGGAGAATA
Hairpin Sequence:
5'-CCGG-TGTTGTCTACCACGGAGAATA-CTCGAG-TATTCTCCGTGGTAGACAACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTTGTCTACCACGGAGAATACTCGAGTATTCTCCGTGGTAGACAACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTTGTCTACCACGGAGAATACTCGAGTATTCTCCGTGGTAGACAACA-3'

Other clones with same target sequence:

(none)