Construct: shRNA TRCN0000361492

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013698.2-1589s21c1
DNA Barcode:
CCTAAAGGCCGTCCGACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Txk (22165)
Gene Description:
TXK tyrosine kinase
Transcript:
RefSeq NM_013698.2 (NON-CURRENT)
Match location:
Position 1589 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361492)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAAAGGCCGTCCGACATTT
Hairpin Sequence:
5'-CCGG-CCTAAAGGCCGTCCGACATTT-CTCGAG-AAATGTCGGACGGCCTTTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAAAGGCCGTCCGACATTTCTCGAGAAATGTCGGACGGCCTTTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAAAGGCCGTCCGACATTTCTCGAGAAATGTCGGACGGCCTTTAGG-3'

Other clones with same target sequence:

(none)