Construct: shRNA TRCN0000361528

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009184.2-1668s21c1
DNA Barcode:
AGAAGAGGGCCTCGTGATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptk6 (20459)
Gene Description:
PTK6 protein tyrosine kinase 6
Transcript:
RefSeq NM_009184.2 (CURRENT)
Match location:
Position 1668 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361528)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGAGGGCCTCGTGATTAT
Hairpin Sequence:
5'-CCGG-AGAAGAGGGCCTCGTGATTAT-CTCGAG-ATAATCACGAGGCCCTCTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGAGGGCCTCGTGATTATCTCGAGATAATCACGAGGCCCTCTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGAGGGCCTCGTGATTATCTCGAGATAATCACGAGGCCCTCTTCT-3'

Other clones with same target sequence:

(none)