Construct: shRNA TRCN0000361580

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011938.3-861s21c1
DNA Barcode:
CCATGGCTCTCAACGAGAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grk6 (26385)
Gene Description:
G protein-coupled receptor kinase 6
Transcript:
RefSeq NM_011938.3 (NON-CURRENT)
Match location:
Position 861 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361580)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGGCTCTCAACGAGAAAC
Hairpin Sequence:
5'-CCGG-CCATGGCTCTCAACGAGAAAC-CTCGAG-GTTTCTCGTTGAGAGCCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGGCTCTCAACGAGAAACCTCGAGGTTTCTCGTTGAGAGCCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGGCTCTCAACGAGAAACCTCGAGGTTTCTCGTTGAGAGCCATGG-3'

Other clones with same target sequence:

(none)