Construct: shRNA TRCN0000361646

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016747.2-2161s21c1
DNA Barcode:
TGGGTTAAGTGACGATTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dlg3 (53310)
Gene Description:
discs, large homolog 3 (Drosophila)
Transcript:
RefSeq NM_016747.2 (NON-CURRENT)
Match location:
Position 2161 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361646)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGGTTAAGTGACGATTATTA
Hairpin Sequence:
5'-CCGG-TGGGTTAAGTGACGATTATTA-CTCGAG-TAATAATCGTCACTTAACCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGGTTAAGTGACGATTATTACTCGAGTAATAATCGTCACTTAACCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGGTTAAGTGACGATTATTACTCGAGTAATAATCGTCACTTAACCCA-3'

Other clones with same target sequence:

(none)