Construct: shRNA TRCN0000361670

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011946.3-897s21c1
DNA Barcode:
GAGTACACAGGCTACTAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Map3k2 (26405)
Gene Description:
mitogen-activated protein kinase kinase kinase 2
Transcript:
RefSeq NM_011946.3 (CURRENT)
Match location:
Position 897 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361670)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTACACAGGCTACTAATTT
Hairpin Sequence:
5'-CCGG-GAGTACACAGGCTACTAATTT-CTCGAG-AAATTAGTAGCCTGTGTACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTACACAGGCTACTAATTTCTCGAGAAATTAGTAGCCTGTGTACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTACACAGGCTACTAATTTCTCGAGAAATTAGTAGCCTGTGTACTC-3'

Other clones with same target sequence:

(none)