Construct: shRNA TRCN0000361704

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020272.2-3990s21c1
DNA Barcode:
CTCGTGTGTCCACCATGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pik3cg (30955)
Gene Description:
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma
Transcript:
RefSeq NM_020272.2 (CURRENT)
Match location:
Position 3990 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361704)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCGTGTGTCCACCATGTATT
Hairpin Sequence:
5'-CCGG-CTCGTGTGTCCACCATGTATT-CTCGAG-AATACATGGTGGACACACGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCGTGTGTCCACCATGTATTCTCGAGAATACATGGTGGACACACGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCGTGTGTCCACCATGTATTCTCGAGAATACATGGTGGACACACGAG-3'

Other clones with same target sequence:

(none)