Construct: shRNA TRCN0000361722

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017469.4-1489s21c1
DNA Barcode:
ACGATCTCTACACCCGATTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gucy1b3 (54195)
Gene Description:
guanylate cyclase 1, soluble, beta 3
Transcript:
RefSeq NM_017469.4 (CURRENT)
Match location:
Position 1489 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361722)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGATCTCTACACCCGATTTG
Hairpin Sequence:
5'-CCGG-ACGATCTCTACACCCGATTTG-CTCGAG-CAAATCGGGTGTAGAGATCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGATCTCTACACCCGATTTGCTCGAGCAAATCGGGTGTAGAGATCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGATCTCTACACCCGATTTGCTCGAGCAAATCGGGTGTAGAGATCGT-3'

Other clones with same target sequence:

(none)