Construct: shRNA TRCN0000361744

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019939.2-1467s21c1
DNA Barcode:
CAGAGATGGAAGCAGATATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mpp6 (56524)
Gene Description:
membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6)
Transcript:
RefSeq NM_019939.2 (CURRENT)
Match location:
Position 1467 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361744)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGATGGAAGCAGATATTA
Hairpin Sequence:
5'-CCGG-CAGAGATGGAAGCAGATATTA-CTCGAG-TAATATCTGCTTCCATCTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGATGGAAGCAGATATTACTCGAGTAATATCTGCTTCCATCTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGATGGAAGCAGATATTACTCGAGTAATATCTGCTTCCATCTCTG-3'

Other clones with same target sequence:

(none)