Construct: shRNA TRCN0000361745

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021028.3-583s21c1
DNA Barcode:
TCTGCGAACCACTCCCGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tk2 (57813)
Gene Description:
thymidine kinase 2, mitochondrial
Transcript:
RefSeq NM_021028.3 (CURRENT)
Match location:
Position 583 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361745)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGCGAACCACTCCCGAAAT
Hairpin Sequence:
5'-CCGG-TCTGCGAACCACTCCCGAAAT-CTCGAG-ATTTCGGGAGTGGTTCGCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGCGAACCACTCCCGAAATCTCGAGATTTCGGGAGTGGTTCGCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGCGAACCACTCCCGAAATCTCGAGATTTCGGGAGTGGTTCGCAGA-3'

Other clones with same target sequence:

(none)