Construct: shRNA TRCN0000361752

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021302.3-1420s21c1
DNA Barcode:
TCCAGCAAGACTTCGTGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stk32c (57740)
Gene Description:
serine/threonine kinase 32C
Transcript:
RefSeq NM_021302.3 (CURRENT)
Match location:
Position 1420 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361752)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCAGCAAGACTTCGTGATTT
Hairpin Sequence:
5'-CCGG-TCCAGCAAGACTTCGTGATTT-CTCGAG-AAATCACGAAGTCTTGCTGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCAGCAAGACTTCGTGATTTCTCGAGAAATCACGAAGTCTTGCTGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCAGCAAGACTTCGTGATTTCTCGAGAAATCACGAAGTCTTGCTGGA-3'

Other clones with same target sequence:

(none)