Construct: shRNA TRCN0000361788

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027539.4-3343s21c1
DNA Barcode:
GAAATGCATTGGACGAATTAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dclk2 (70762)
Gene Description:
doublecortin-like kinase 2
Transcript:
RefSeq NM_027539.4 (NON-CURRENT)
Match location:
Position 3343 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361788)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAATGCATTGGACGAATTAG
Hairpin Sequence:
5'-CCGG-GAAATGCATTGGACGAATTAG-CTCGAG-CTAATTCGTCCAATGCATTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAATGCATTGGACGAATTAGCTCGAGCTAATTCGTCCAATGCATTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAATGCATTGGACGAATTAGCTCGAGCTAATTCGTCCAATGCATTTC-3'

Other clones with same target sequence:

(none)