Construct: shRNA TRCN0000361884

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023815.4-1211s21c1
DNA Barcode:
GGTGATTAGGCTTGGCCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trp53rkb (76367)
Gene Description:
transformation related protein 53 regulating kinase B
Transcript:
RefSeq NM_023815.4 (CURRENT)
Match location:
Position 1211 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361884)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTGATTAGGCTTGGCCATAA
Hairpin Sequence:
5'-CCGG-GGTGATTAGGCTTGGCCATAA-CTCGAG-TTATGGCCAAGCCTAATCACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTGATTAGGCTTGGCCATAACTCGAGTTATGGCCAAGCCTAATCACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTGATTAGGCTTGGCCATAACTCGAGTTATGGCCAAGCCTAATCACC-3'

Other clones with same target sequence:

(none)