Construct: shRNA TRCN0000361945

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012040.2-470s21c1
DNA Barcode:
CGCTGTCTCCTACCTTCATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pnck (93843)
Gene Description:
pregnancy upregulated non-ubiquitously expressed CaM kinase
Transcript:
RefSeq NM_012040.2 (NON-CURRENT)
Match location:
Position 470 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361945)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGTCTCCTACCTTCATAG
Hairpin Sequence:
5'-CCGG-CGCTGTCTCCTACCTTCATAG-CTCGAG-CTATGAAGGTAGGAGACAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGTCTCCTACCTTCATAGCTCGAGCTATGAAGGTAGGAGACAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGTCTCCTACCTTCATAGCTCGAGCTATGAAGGTAGGAGACAGCG-3'

Other clones with same target sequence:

(none)