Construct: shRNA TRCN0000361994

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144817.2-514s21c1
DNA Barcode:
AGGTCTTGTCTGCGGTGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Camk1g (215303)
Gene Description:
calcium/calmodulin-dependent protein kinase I gamma
Transcript:
RefSeq NM_144817.2 (CURRENT)
Match location:
Position 514 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000361994)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGTCTTGTCTGCGGTGAAAT
Hairpin Sequence:
5'-CCGG-AGGTCTTGTCTGCGGTGAAAT-CTCGAG-ATTTCACCGCAGACAAGACCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGTCTTGTCTGCGGTGAAATCTCGAGATTTCACCGCAGACAAGACCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGTCTTGTCTGCGGTGAAATCTCGAGATTTCACCGCAGACAAGACCT-3'

Other clones with same target sequence:

(none)