Construct: shRNA TRCN0000362176

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198191.2-708s21c1
DNA Barcode:
ACATCAAGGGCTGCAACATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pip5kl1 (227733)
Gene Description:
phosphatidylinositol-4-phosphate 5-kinase-like 1
Transcript:
RefSeq NM_198191.2 (NON-CURRENT)
Match location:
Position 708 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362176)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACATCAAGGGCTGCAACATAA
Hairpin Sequence:
5'-CCGG-ACATCAAGGGCTGCAACATAA-CTCGAG-TTATGTTGCAGCCCTTGATGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACATCAAGGGCTGCAACATAACTCGAGTTATGTTGCAGCCCTTGATGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACATCAAGGGCTGCAACATAACTCGAGTTATGTTGCAGCCCTTGATGT-3'

Other clones with same target sequence:

(none)