Construct: shRNA TRCN0000362191

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172734.2-455s21c1
DNA Barcode:
TCCGAGCAGAAAGGGACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stk38l (232533)
Gene Description:
serine/threonine kinase 38 like
Transcript:
RefSeq NM_172734.2 (NON-CURRENT)
Match location:
Position 455 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362191)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCGAGCAGAAAGGGACATTT
Hairpin Sequence:
5'-CCGG-TCCGAGCAGAAAGGGACATTT-CTCGAG-AAATGTCCCTTTCTGCTCGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCGAGCAGAAAGGGACATTTCTCGAGAAATGTCCCTTTCTGCTCGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCGAGCAGAAAGGGACATTTCTCGAGAAATGTCCCTTTCTGCTCGGA-3'

Other clones with same target sequence:

(none)