Construct: shRNA TRCN0000362199

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146191.3-2880s21c1
DNA Barcode:
GCAGACGCCTGGCAATGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrrk1 (233328)
Gene Description:
leucine-rich repeat kinase 1
Transcript:
RefSeq NM_146191.3 (CURRENT)
Match location:
Position 2880 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362199)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGACGCCTGGCAATGATAT
Hairpin Sequence:
5'-CCGG-GCAGACGCCTGGCAATGATAT-CTCGAG-ATATCATTGCCAGGCGTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGACGCCTGGCAATGATATCTCGAGATATCATTGCCAGGCGTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGACGCCTGGCAATGATATCTCGAGATATCATTGCCAGGCGTCTGC-3'

Other clones with same target sequence:

(none)