Construct: shRNA TRCN0000362229

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146239.2-1643s21c1
DNA Barcode:
ACAAGCCACAACCTCTAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdk17 (237459)
Gene Description:
cyclin-dependent kinase 17
Transcript:
RefSeq NM_146239.2 (CURRENT)
Match location:
Position 1643 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362229)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAAGCCACAACCTCTAATTA
Hairpin Sequence:
5'-CCGG-ACAAGCCACAACCTCTAATTA-CTCGAG-TAATTAGAGGTTGTGGCTTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAAGCCACAACCTCTAATTACTCGAGTAATTAGAGGTTGTGGCTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAAGCCACAACCTCTAATTACTCGAGTAATTAGAGGTTGTGGCTTGT-3'

Other clones with same target sequence:

(none)