Construct: shRNA TRCN0000362255

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172911.3-1674s21c1
DNA Barcode:
AGAGGACCACAGGACTATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prag1 (244418)
Gene Description:
PEAK1 related kinase activating pseudokinase 1
Transcript:
RefSeq NM_172911.3 (CURRENT)
Match location:
Position 1674 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362255)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGGACCACAGGACTATTTA
Hairpin Sequence:
5'-CCGG-AGAGGACCACAGGACTATTTA-CTCGAG-TAAATAGTCCTGTGGTCCTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGGACCACAGGACTATTTACTCGAGTAAATAGTCCTGTGGTCCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGGACCACAGGACTATTTACTCGAGTAAATAGTCCTGTGGTCCTCT-3'

Other clones with same target sequence:

(none)