Construct: shRNA TRCN0000362323

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153744.3-1756s21c1
DNA Barcode:
AGTGCACCAGGACGATGAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prkag3 (241113)
Gene Description:
protein kinase, AMP-activated, gamma 3 non-catatlytic subunit
Transcript:
RefSeq NM_153744.3 (CURRENT)
Match location:
Position 1756 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362323)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGCACCAGGACGATGAAAG
Hairpin Sequence:
5'-CCGG-AGTGCACCAGGACGATGAAAG-CTCGAG-CTTTCATCGTCCTGGTGCACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGCACCAGGACGATGAAAGCTCGAGCTTTCATCGTCCTGGTGCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGCACCAGGACGATGAAAGCTCGAGCTTTCATCGTCCTGGTGCACT-3'

Other clones with same target sequence:

(none)