Construct: shRNA TRCN0000362333

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172911.3-4474s21c1
DNA Barcode:
ACCCTGGGAAATGGTACAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prag1 (244418)
Gene Description:
PEAK1 related kinase activating pseudokinase 1
Transcript:
RefSeq NM_172911.3 (CURRENT)
Match location:
Position 4474 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362333)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCTGGGAAATGGTACAAAT
Hairpin Sequence:
5'-CCGG-ACCCTGGGAAATGGTACAAAT-CTCGAG-ATTTGTACCATTTCCCAGGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCTGGGAAATGGTACAAATCTCGAGATTTGTACCATTTCCCAGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCTGGGAAATGGTACAAATCTCGAGATTTGTACCATTTCCCAGGGT-3'

Other clones with same target sequence:

(none)