Construct: shRNA TRCN0000362416

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172729.2-2946s21c1
DNA Barcode:
GAACCAGACGCTACGGCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nod1 (107607)
Gene Description:
nucleotide-binding oligomerization domain containing 1
Transcript:
RefSeq NM_172729.2 (NON-CURRENT)
Match location:
Position 2946 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362416)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCAGACGCTACGGCATTT
Hairpin Sequence:
5'-CCGG-GAACCAGACGCTACGGCATTT-CTCGAG-AAATGCCGTAGCGTCTGGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCAGACGCTACGGCATTTCTCGAGAAATGCCGTAGCGTCTGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCAGACGCTACGGCATTTCTCGAGAAATGCCGTAGCGTCTGGTTC-3'

Other clones with same target sequence:

(none)