Construct: shRNA TRCN0000362428

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008138.4-1041s21c1
DNA Barcode:
AGCAAGTTTGAGGATCTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gnai2 (14678)
Gene Description:
guanine nucleotide binding protein (G protein), alpha inhibiting 2
Transcript:
RefSeq NM_008138.4 (NON-CURRENT)
Match location:
Position 1041 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362428)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAAGTTTGAGGATCTAAAT
Hairpin Sequence:
5'-CCGG-AGCAAGTTTGAGGATCTAAAT-CTCGAG-ATTTAGATCCTCAAACTTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAAGTTTGAGGATCTAAATCTCGAGATTTAGATCCTCAAACTTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAAGTTTGAGGATCTAAATCTCGAGATTTAGATCCTCAAACTTGCT-3'

Other clones with same target sequence:

(none)