Construct: shRNA TRCN0000362431

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175088.5-1292s21c1
DNA Barcode:
GCCGGATGTATGTGGTTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mdfic (16543)
Gene Description:
MyoD family inhibitor domain containing
Transcript:
RefSeq NM_175088.5 (CURRENT)
Match location:
Position 1292 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362431)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGGATGTATGTGGTTTAAT
Hairpin Sequence:
5'-CCGG-GCCGGATGTATGTGGTTTAAT-CTCGAG-ATTAAACCACATACATCCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGGATGTATGTGGTTTAATCTCGAGATTAAACCACATACATCCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGGATGTATGTGGTTTAATCTCGAGATTAAACCACATACATCCGGC-3'

Other clones with same target sequence:

(none)