Construct: shRNA TRCN0000362466

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201519.2-721s21c1
DNA Barcode:
TCACGTGACTGGGCCATTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Map4k5 (399510)
Gene Description:
mitogen-activated protein kinase kinase kinase kinase 5
Transcript:
RefSeq NM_201519.2 (CURRENT)
Match location:
Position 721 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362466)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCACGTGACTGGGCCATTATC
Hairpin Sequence:
5'-CCGG-TCACGTGACTGGGCCATTATC-CTCGAG-GATAATGGCCCAGTCACGTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCACGTGACTGGGCCATTATCCTCGAGGATAATGGCCCAGTCACGTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCACGTGACTGGGCCATTATCCTCGAGGATAATGGCCCAGTCACGTGA-3'

Other clones with same target sequence:

(none)