Construct: shRNA TRCN0000362585

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009829.3-1482s21c1
DNA Barcode:
CTGCAACATGGGAACGAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccnd2 (12444)
Gene Description:
cyclin D2
Transcript:
RefSeq NM_009829.3 (CURRENT)
Match location:
Position 1482 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362585)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCAACATGGGAACGAATTA
Hairpin Sequence:
5'-CCGG-CTGCAACATGGGAACGAATTA-CTCGAG-TAATTCGTTCCCATGTTGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCAACATGGGAACGAATTACTCGAGTAATTCGTTCCCATGTTGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCAACATGGGAACGAATTACTCGAGTAATTCGTTCCCATGTTGCAG-3'

Other clones with same target sequence:

(none)