Construct: shRNA TRCN0000362606

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031185.3-552s21c1
DNA Barcode:
AGGAGGAAACACCGGAAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Akap12 (83397)
Gene Description:
A kinase (PRKA) anchor protein (gravin) 12
Transcript:
RefSeq NM_031185.3 (CURRENT)
Match location:
Position 552 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362606)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAGGAAACACCGGAAATAA
Hairpin Sequence:
5'-CCGG-AGGAGGAAACACCGGAAATAA-CTCGAG-TTATTTCCGGTGTTTCCTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAGGAAACACCGGAAATAACTCGAGTTATTTCCGGTGTTTCCTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAGGAAACACCGGAAATAACTCGAGTTATTTCCGGTGTTTCCTCCT-3'

Other clones with same target sequence:

(none)