Construct: shRNA TRCN0000362661

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008540.2-916s21c1
DNA Barcode:
ATGAGTACGTTCACGACTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smad4 (17128)
Gene Description:
SMAD family member 4
Transcript:
RefSeq NM_008540.2 (NON-CURRENT)
Match location:
Position 916 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362661)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGAGTACGTTCACGACTTTG
Hairpin Sequence:
5'-CCGG-ATGAGTACGTTCACGACTTTG-CTCGAG-CAAAGTCGTGAACGTACTCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGAGTACGTTCACGACTTTGCTCGAGCAAAGTCGTGAACGTACTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGAGTACGTTCACGACTTTGCTCGAGCAAAGTCGTGAACGTACTCAT-3'

Other clones with same target sequence:

(none)