Construct: shRNA TRCN0000362703

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194462.2-4860s21c1
DNA Barcode:
CAAGAACTTGTGCGACAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Akap9 (100986)
Gene Description:
A kinase (PRKA) anchor protein (yotiao) 9
Transcript:
RefSeq NM_194462.2 (CURRENT)
Match location:
Position 4860 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362703)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAACTTGTGCGACAATAT
Hairpin Sequence:
5'-CCGG-CAAGAACTTGTGCGACAATAT-CTCGAG-ATATTGTCGCACAAGTTCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAACTTGTGCGACAATATCTCGAGATATTGTCGCACAAGTTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAACTTGTGCGACAATATCTCGAGATATTGTCGCACAAGTTCTTG-3'

Other clones with same target sequence:

(none)