Construct: shRNA TRCN0000362715

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021878.2-3901s21c1
DNA Barcode:
GCCGAACCCATGGTCACTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Jarid2 (16468)
Gene Description:
jumonji, AT rich interactive domain 2
Transcript:
RefSeq NM_021878.2 (NON-CURRENT)
Match location:
Position 3901 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362715)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAACCCATGGTCACTTAT
Hairpin Sequence:
5'-CCGG-GCCGAACCCATGGTCACTTAT-CTCGAG-ATAAGTGACCATGGGTTCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAACCCATGGTCACTTATCTCGAGATAAGTGACCATGGGTTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAACCCATGGTCACTTATCTCGAGATAAGTGACCATGGGTTCGGC-3'

Other clones with same target sequence:

(none)