Construct: shRNA TRCN0000362740

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009399.3-132s21c1
DNA Barcode:
TGCACCCAGGAGAGGCATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnfrsf11a (21934)
Gene Description:
tumor necrosis factor receptor superfamily, member 11a, NFKB activator
Transcript:
RefSeq NM_009399.3 (CURRENT)
Match location:
Position 132 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362740)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCACCCAGGAGAGGCATTAT
Hairpin Sequence:
5'-CCGG-TGCACCCAGGAGAGGCATTAT-CTCGAG-ATAATGCCTCTCCTGGGTGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCACCCAGGAGAGGCATTATCTCGAGATAATGCCTCTCCTGGGTGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCACCCAGGAGAGGCATTATCTCGAGATAATGCCTCTCCTGGGTGCA-3'

Other clones with same target sequence:

(none)