Construct: shRNA TRCN0000362814

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019483.4-1663s21c1
DNA Barcode:
ATCGTGGCAACCGCAGCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smad9 (55994)
Gene Description:
SMAD family member 9
Transcript:
RefSeq NM_019483.4 (NON-CURRENT)
Match location:
Position 1663 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362814)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCGTGGCAACCGCAGCTAAT
Hairpin Sequence:
5'-CCGG-ATCGTGGCAACCGCAGCTAAT-CTCGAG-ATTAGCTGCGGTTGCCACGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCGTGGCAACCGCAGCTAATCTCGAGATTAGCTGCGGTTGCCACGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCGTGGCAACCGCAGCTAATCTCGAGATTAGCTGCGGTTGCCACGAT-3'

Other clones with same target sequence:

(none)