Construct: shRNA TRCN0000362922

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009397.2-1366s21c1
DNA Barcode:
AGCTATCACTCATGGATATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnfaip3 (21929)
Gene Description:
tumor necrosis factor, alpha-induced protein 3
Transcript:
RefSeq NM_009397.2 (NON-CURRENT)
Match location:
Position 1366 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362922)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTATCACTCATGGATATAA
Hairpin Sequence:
5'-CCGG-AGCTATCACTCATGGATATAA-CTCGAG-TTATATCCATGAGTGATAGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTATCACTCATGGATATAACTCGAGTTATATCCATGAGTGATAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTATCACTCATGGATATAACTCGAGTTATATCCATGAGTGATAGCT-3'

Other clones with same target sequence:

(none)