Construct: shRNA TRCN0000362941

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172430.3-1820s21c1
DNA Barcode:
CACCTTCTACAACCGAGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sphkap (77629)
Gene Description:
SPHK1 interactor, AKAP domain containing
Transcript:
RefSeq NM_172430.3 (CURRENT)
Match location:
Position 1820 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362941)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCTTCTACAACCGAGTATT
Hairpin Sequence:
5'-CCGG-CACCTTCTACAACCGAGTATT-CTCGAG-AATACTCGGTTGTAGAAGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCTTCTACAACCGAGTATTCTCGAGAATACTCGGTTGTAGAAGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCTTCTACAACCGAGTATTCTCGAGAATACTCGGTTGTAGAAGGTG-3'

Other clones with same target sequence:

(none)