Construct: shRNA TRCN0000362953

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001163175.1-158s21c1
DNA Barcode:
GCTCTCCTACACCACGCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Begain (380785)
Gene Description:
brain-enriched guanylate kinase-associated
Transcript:
RefSeq NM_001163175.1 (CURRENT)
Match location:
Position 158 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000362953)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTCCTACACCACGCATAA
Hairpin Sequence:
5'-CCGG-GCTCTCCTACACCACGCATAA-CTCGAG-TTATGCGTGGTGTAGGAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTCCTACACCACGCATAACTCGAGTTATGCGTGGTGTAGGAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTCCTACACCACGCATAACTCGAGTTATGCGTGGTGTAGGAGAGC-3'

Other clones with same target sequence:

(none)